Research

Due to maternity leave we had to take a break in my lab. We are starting back up this spring with a project involving urinary tract infections at a local hospital as well as exploring  projects in marine microbial ecology.

Current and Past Research Projects: 

Antibiotic resistances in hospital isolated bacteria from urinary tract infections (UTIs) UTIs  occur when infectious bacteria enters the urinary tract typically through the urethra and infect the bladder. Approximately 10 million doctor visits a year are due to UTIs with 80 percent of those cases occurring in women. The most common causative agent of a UTI in the United States is Escherichia coli. This is followed by Klebsiella spp, and Proteus mirabilis. Currently, the Centers for Disease Control recommends antibiotics for treating a UTI. The sulfonamide, Trimethoprim/sulfamethoxazole (TMP/Sulfa) and the nitrofuran, nitrofuratonin, are most commonly prescribed for UTI treatment nationwide.  Due to antibiotic use and recurrent UTIs, many etiological agents are becoming antibiotic resistant. We are investigating hospital isolates to identify common strains of bacteria that cause UTIs and the mode of antibiotic resistance these microbes utilize.

Slide1

RNA Methyltransferase Gene Identification in Neisseria gonorrhea Isolates The Centers for Disease Control designated Neisseria gonorrhea, the etiological agent of the common sexually transmitted disease gonorrhea, as a superbug. The agency currently recommends the dual therapy of 250 mg of intramuscular ceftriaxone and 1 g of oral azithromycin. Erythromycin therapy was discontinued in the early 1990’s due to widespread resistance. This study is aimed at identifying the presence of erythromycin resistance-inducing methyltransferase genes ermA, ermB, ermC, and ermF in current clinical isolates

.Slide1

Publications: 

Hollander, A.M, Straub, D. Willis, J. The evaluation of active learning techniques for the review of basic sciences and the impact of these methods by multiple choice exam assessment In preparation

Gregory A.Price, Aimee M. Hollander, Brian D. Plikaytis, Brian T. Mocca, George Carlone, Helen Findlow, Ray Borrow, Samba O. Sow, Aldiouma Diallo, Olubukola T. Idoko, Godwin C. Enwere, Marie-Pierre Preziosi; Prasad S. Kulkarni, Margaret C. Bash, Human complement bactericidal responses to a Group A meningococcal conjugate vaccine in Africans and comparison to responses measured by two other group A immunoassays 2015, November 15 Clinical Infectious Disease 61 (suppl 5): S554-S562 doi:10.1093/cid/civ504

Hollander, A.M., Mercante, A., Shafer W.M., and Corneliessen, C.N.: MpeR, an AraC-like regulator activates FetA expression in strain FA1090, Infection and Immunity 2011 Dec;79(12):4764-76.

Cornelissen, C.N, and Hollander, A.M: TonB-dependent transporters expressed by Neisseria gonorrhoeae, Frontiers in Microbiology, 2011 http://www.frontiersin.org/Journal/Abstract.aspx?s=149&name=cellular and infection microbiology&ART_DOI=10.3389/fmicb.2011.00117

Price, G.A., Masri H.P., Hollander, A.M., Russell, M.W., and Cornelissen, C.N.: Gonoccocal transferrin binding protein-cholera toxin B chimeras induce serum bactericidal and vaginal transferrin utilizations blocking antibodies in mice, Vaccine, 25 (41): 7247-7260, 2007.

Delboy, M.G., Patterson, J.L., Hollander, A.M., Nicola A.V.: Nectin-2-mediated entry of a syncytial strain of herpes simplex virus via pH- independent fusion with the plasma membrane of Chinese hamster ovary cells, Virology Journal, 2006 Dec 27;3:105

 

 Presentations: 

RNA Methyltransferase Gene Identification in Neisseria gonorrhea Isolates Collected from Thibodaux Regional Medical Center January 2016 to September 2016 Brendon J. Gros1, Anne Guidry2, Wendy Toup2, Aimee M Hollander 1 American Society of Microbiology South Central Branch Meeting, October 28-29th, 2016, The University of Louisiana Lafayette (Poster)

Antibiotic Resistance in Bacteria Isolated from Urinary Tract Infections at Thibodaux Regional Medical Center from July 2015-July 2016 Dexter Hebert1, Wendy Toups2, Aimee M Hollander1 American Society of Microbiology South Central Branch Meeting, October 28-29th, 2016, The University of Louisiana Lafayette (Poster)

 

A.M. Hollander1, H.F. Nawar1, A. Cohn2, M.C. Bash1
Development of an intrinsic killing assay for identifying susceptible individuals to Neisseria meningitidis serogroup B strains 18th International Pathogenic Neisseria Conference September 4th-9th 2012, Wurzburg, German (Poster)

Aimee Hollander1, Alexandra Mercante2, William M. Shafer2, Cynthia Nau Cornelissen1 (2010) Gonococcal FetA Expression is Regulated by MpeR and Enables Strain FA1090 to Utilize Enterobactin and its Linear Derivatives. 17th International Pathogenic Neisseria Conference September 11th- 16th 2010, Banff, Alberta Canada (Poster)

Aimee Hollander1, Alexandra Mercante2, Lydgia Jackson3, Cynthia Nau Cornelissen1, William M. Shafer2, David Dyer3, (2010) The AraC-like Transcriptional Regulator, MpeR, Activates Expression of FetA in Neisseria gonorrhoeae. American Society of Microbiology 110th General Meeting May 23rd-27th, San Diego, CA (Poster)

Aimee Hollander1, Cynthia Nau Cornelissen1, (2009) The role of Mper, an AraC-like Regulator and Iron on the Expression of Outer Membrane Proteins in Neisseria gonorrhoeae. American Society of Microbiology, Virginia Branch Meeting, November 13th-14th, Richmond, VA. (Oral presentation)

 

Past and Present Lab Members 

BJ Gross- currently studying medicine at LSUHSC NOLA

Dexter Hebert – Currently studying Optometry at UAB